Figure 4B shows results using challenge strain CL-10 (2.1 X 10 8 CFU/ml). The polypeptide of SEQ ID NO: 28 was used as immunogen Figure 4A shows results using challenge strain CL-10 (2.2 X 10 8 CFU/ml). Figures 4A-4H illustrates the ability of ORF0657n related polypeptides to provide protective immunity against different S.Reference to a "carboxyl His-Tag" indicates the His-Tag group LEHHHHHH (SEQ ID NO: 64) is present at the carboxyl terminus.
Figure 3C illustrates results with SEQ ID NO: 5 containing a carboxyl His-Tag. Figure 3B illustrates results with SEQ ID NO: 4 containing a carboxyl His-Tag. Figure 3A illustrates results with SEQ ID NO: 28. The polypeptides were used with an aluminum hydroxyphosphate adjuvant (AHP).
SEQ ID NO: 28 contains a carboxyl "His-Tag" (LEHHHHHH SEQ ID NO: 64). Figure 1B provides a full-length sequence used as a reference for Figure 1A (SEQ ID NO: 2). Figure 1A illustrates a schematic of polypeptides that were tested and found protective (shown by filled in rectangles), polypeptides tested and found not to be protective (shown by open rectangles), and a polypeptide not tested (hatched box). Figures 1A, 1B, 1C, and 1D provide a schematic illustrating ORF0657n related polypeptide regions screened for protection in animals, and some different ORF0657n sequences.
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